게시판
| 21KS-042 |
The changing trend of serum Neuron Specific Enolase in the Partial Sciatic Nerve Ligation model of mouse
울산대학교 의과대학 마취통증의학교실
Background: Enolase is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase exists in the form of several tissue-specific isoenzymes (alpha, beta, and gamma). Neuron specific enolase (NSE) is a gamma isoenzyme and represents the dominant enolase-isoenzyme found in neuronal and neuroendocrine tissues. NSE is also frequently overexpressed by neural crest-derived tumors, such as small cell lung carcinoma, carcinoids, islet cell tumors, and neuroblastoma, etc. High levels have therefore some negative prognostic value. Falling or rising levels are often correlated with tumor shrinkage or recurrence, respectively. Due to this organ-specificity, concentrations of NSE in serum are often elevated in diseases that result in relative rapid neuronal destruction. Measurement of NSE in serum can therefore assist in the differential diagnosis of a variety of neuron-destructive and neurodegenerative disorders. The most common application is in the differential diagnosis of dementias where elevated CSF concentrations support the diagnosis of rapidly progressive dementias,
There is increasing evidence that elevated serum NSE levels correlate with a poor outcome in coma, particularly when caused by hypoxic insult.
However, the changing trends of serum NSE was not evaluated in the pain models. We hypopthesized that serum NSE concentrations could be elevated after nerve injury model as time goes on. In the present study, it was investigated the changing trends of serum NSE concentrations in the PSNL model of mouse
Methods: The experiments were conducted in male ICR mice (25-35 g).
Neuropathic pain was induced by partial ligation of the left sciatic nerve. For this, mice were initially anesthetized with isoflurane (2%atic nerve was exposed at midthigh level under aseptic condition. One-half to one-third of the nerve just proximal to the trifurcation was ligated with one tight ligation using a medical polypropylene thread (9ongly sutured with polypropylene threads (7operation was performed in parallel in the control group mice by exposing the left sciatic nerve and then closing the wound without ligation.
Results: Allodynua was produced after PSNL.
The Serum NSE concentrations abruptly elevated at the 3h and 5h time points. After then it gradually decreased to 5d time point. On the 5d time point, the serum NSE concentrations were significantly higher than the baseline level of NSE concentration.
Conclusions: NSE might have utility as a prognostic marker for the diagnosis or treatment in neuronal injury or neuropathic pain.
There is increasing evidence that elevated serum NSE levels correlate with a poor outcome in coma, particularly when caused by hypoxic insult.
However, the changing trends of serum NSE was not evaluated in the pain models. We hypopthesized that serum NSE concentrations could be elevated after nerve injury model as time goes on. In the present study, it was investigated the changing trends of serum NSE concentrations in the PSNL model of mouse
Methods: The experiments were conducted in male ICR mice (25-35 g).
Neuropathic pain was induced by partial ligation of the left sciatic nerve. For this, mice were initially anesthetized with isoflurane (2%atic nerve was exposed at midthigh level under aseptic condition. One-half to one-third of the nerve just proximal to the trifurcation was ligated with one tight ligation using a medical polypropylene thread (9ongly sutured with polypropylene threads (7operation was performed in parallel in the control group mice by exposing the left sciatic nerve and then closing the wound without ligation.
Results: Allodynua was produced after PSNL.
The Serum NSE concentrations abruptly elevated at the 3h and 5h time points. After then it gradually decreased to 5d time point. On the 5d time point, the serum NSE concentrations were significantly higher than the baseline level of NSE concentration.
Conclusions: NSE might have utility as a prognostic marker for the diagnosis or treatment in neuronal injury or neuropathic pain.